为了深入了解潜伏感染的细胞,作者研究了hiv感染的病毒血症患者、正在接受抗病毒治疗的患者以及对照人群的hiv病毒的基因组整合谱
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lillian b. cohn, israel t. silva, thiago y. oliveira, rafael a. rosales, erica h. parrish, gerald h. learn, beatrice h. hahn, julie l. czartoski, m. juliana mcelrath, clara lehmann, florian klein, marina艾滋病潜伏期多久 caskey, bruce d. walker, janet d. siliciano, robert f. siliciano, mila jankovic, michel c. nussenzweig
the barrier to curing hiv-1 is thought to reside primarily in cd4+ t cells containing silent proviruses. to characterize these latently infected cells, we studied the integration profile of hiv-1 in v接吻艾滋病传播途径iremic progressors, individuals receiving antiretroviral therapy, and viremic controllers. clonally expanded t cells represented the majority of all integrations and increased during therapy. however, none of the 75 expanded t cell clones assayed contained intact virus. in contrast, the cells bearing 男性艾滋病初期症状single integration events decreased in frequency over time on therapy, and the surviving cells were enriched for hiv-1 integration in silent regions of the genome. finally, there was a strong preference for integration into, or in close proximity to, alu repeats, which were also enriched in local hot艾滋病最早期症状spots for integration. the data indicate that dividing clonally expanded t cells contain defective proviruses and that the replication-competent reservoir is primarily found in cd4+ t cells that remain relatively quiescent.
2015年2月12日 讯/生物谷bioon/--hiv是一种人类免疫缺陷病毒,能够摧毁人体免疫系统,导致各种疾病及癌症得以在人体内生存,最终导致艾滋病而使患者死亡目前尚无艾滋病潜伏期多久有效的治愈艾滋病的方法近日,nussenzweig团队发现hiv更容易在安静的环境中潜伏生存,这一发现使人类在攻克艾滋病的征途中又迈进了重要的一步
hiv-1 integration landscape during latent and active infection
doi: http://dx.doi.org/10.1016/j.cell.2015.01.020
hiv可与淋巴细胞表面的cd4分子结合进入淋巴细胞,从而破坏免疫系统hiv感染人体后,常被划分为四个明显的阶段:急性感染期、潜伏期、艾滋病前期和典型艾滋病期患者在hiv感染后无法治愈的主要原因是cd4+t细胞中存在静息态的前病毒结果表明,大部分整合均发生在克隆扩增的t艾滋病初期症状细胞中,且随着抗病毒治疗这些细胞也出现增多但是,在所有75个扩增的t细胞克隆中,没有一个克隆包含完整的病毒相比之下,包含单点整合的细胞随着治疗逐渐减少,存活下来的细胞中hiv整合的位点均在基因组的沉默区域然而,整合通常发生在高频插入靶点"alu重复"中或该靶点附近此外,实验数据显示,正在分裂的克隆扩增的t细胞内会含缺陷的前病毒,但是增殖型的病毒主要存在于相对静息态的cd4+t细胞中可见,hiv更容易待在安静的环境中潜伏(生物谷bioon.com)
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